中国 Wuhan Desheng Biochemical Technology Co., Ltd 企業ニュース

DNAの核酸の電気泳動は核酸PCRの重要なステップ、分離および浄化、核酸の検出および他のテストです。アガロースのゲルの電気泳動のキットは核酸の電気泳動を促進するために開発される電気泳動のキット プロダクトです。従来のゲルの電気泳動と比較されて多くの利点を持っています。 アガロースのゲル、電気泳動の液体およびローディングの緩衝   電気泳動は電界の行為の下で電気特性と反対に電極の方に荷電粒子の動きを示します。特定の条件下で、荷電粒子の移動率（移動性）は固定です。DNAの電気泳動またはサザンブロッティングのしみの交配では、荷電粒子は核酸DNAを示し、別のDNAsに互いとは別に異なった移動性がおよびこうしてあります。核酸の粒子がpHに非常に敏感であるので、緩衝は核酸への電気泳動の解決に加えられなければなりません。緩衝部品は緩衝に荷を積むアガロースのゲル、TAEまたはTBEの電気泳動の解決で含まれています。   通常、アガロースのゲルの電気泳動は次のステップによって行く必要があります:アガロースの電気泳動のゲルの準備、電気泳動の解決の準備、電気泳動タンクの偵察、電気泳動およびクリーニング。その中で、長い時間はアガロースのゲルの準備です。それは混合の解決を準備し、アガロースを重量を量り、電子レンジで溶け、解決を60度に冷却し、冷却するためにゲルを注ぎ装置をきれいにする必要があります。プロセスは非常に扱いにくく、たくさん繰り返されて、1つを取ります| 1.5時間。アガロースのゲルの電気泳動のキットは異なっています: 1. アガロース、核酸の染料、電気泳動の解決およびローディングの緩衝のような試薬を購入する必要性がありません。 2. ゲルの作成のcumbersomenessを除去すれば、前作られたゲルは汚れる電気泳動の解決のための核酸の染料、必要性または後汚損と前汚れません。簡単および便利、使用可能。核酸の無駄を減らす前作られたゲルを使用するときDNAのサンプルまたは電気泳動の解決の核酸の染料を加える必要性は染まらないし、前汚されたゲルの極端に低い毒性はオペレータのために大いに安全より直接使用します核酸の染料をです。 3. キットは高圧速い電気泳動の解決が特に装備されています。あなたの核酸のサンプル片が2000bpの下にあれば、電気泳動の速度をいつでも制御し、電圧を調節できます。一般的な小型ゲルは最も速いのの5-10分に完了することができます;マーカーまたは核酸のサンプルに多くのバンドがあり、長い片（核酸のサンプル片は2000bpより大きいです）、適した低電圧に合わせることができるまたはあなたはまだあなたの元の低電圧の電気泳動の状態を使用できます。 4. このプロダクトの電気泳動はそれに続く南交配に影響を与えないし、得られたDNAの片はゲルの回復に服従し、それに続くDNAのligationおよび他の反作用は影響を受けていません。   つまり、従来の核酸の電気泳動方法と比較されて、アガロースによってプレキャストされるゲルの電気泳動のキットにセービングの時間、セービングの労働、高圧、速い、およびセービングの費用の利点があります。それは強くDeshengによって推薦されるプロダクトです。当然、TAEのTBEの電気泳動のキットがありますまたはTrisの緩衝他のまた私達の会社に連絡できます。

Virus Transport Media is divided into inactivated type and activated type. It is a solution that protects the head of the virus swab after sampling in the virus sampling tube, which can prevent the swab from being immediately after the virus sampling In the case of detection, it can also be stored or transported for a period of time to prevent the viral nucleic acid from being decomposed and undetectable.   There are many steps to detect the entire viral nucleic acid. Among them, sampling with nasopharyngeal swabs or other swabs, as well as the storage and transportation of viral samples are the pre-processing steps of nucleic acid detection. Swabs are a more commonly used method of taking biological samples, and can be used for molecular biology analysis such as PCR and nucleic acid detection. The characteristics of swab sampling are fast and non-intrusive, which will not cause harm or other impact to the sampled object. It is very suitable for large-scale screening sampling and sampling of special groups (such as children and the elderly) and tissues and organs.   Since most virus sampling sites do not have the conditions for immediate detection, it is important to store and transport the virus for inspection, and the virus is difficult to survive in vitro, so it is necessary to use the virus transport media The virus sample soaked up. Among them, the inactivated virus transport media is safer, and conventional cryopreservation is sufficient. The samples stored in the activated virus transport media have shorter storage time or require strict cryopreservation, but the detection rate is higher, and not only can be used for nucleic acids Detection. However, it should be noted that the more virus transport media in the sampling tube, the better the preservation. Because the virus transport media is a solution, it will have a dilution effect on the virus sample. Too much addition will reduce the detection rate of nucleic acid detection.   After the virus sample is delivered to the testing institution, the nucleic acid is purified through the processes of lysate lysis, centrifugation, separation, etc. When extracting DNA or RNA, care should be taken to prevent the cleavage of the nucleic acid. RNA samples also need to undergo reverse transcription reactions through reverse transcription primers, dNTPs, reverse transcriptase, etc. to produce the corresponding DNA. Then, the DNA polymerase catalyzes PCR amplification with specific primers of viral cDNA. If there are amplified DNA bands, it can be determined that the sample contains virus, otherwise it is not.   Virus transport media, sampling swabs, and sampling tubes related to virus detection are the products recommended by Desheng. Especially in the case of serious epidemics, it is also the company's purpose to provide high-quality goods for related products in short supply in the market. Large quantities of preservatives and swabs can also be discounted.

Due to the New coronavirus epidemic, Our work and life have been greatly affected. The detection of biological viruses and nucleic acids has also become well known from the public, and the corresponding virus sampling and virus transport media has also become a kind of biological reagent raw material with great demand in the market. There is a huge demand for a raw material of biological reagents. Of course, many people are curious about how it is made.   According to the different functions of virus transport media, there are two types of inactivated and activated types, of course, the production method is also different. This article focuses on the inactivated virus transport media. The biggest difference between the inactivated and activated types is that the inactivated type does not need to maintain the integrity of the virus structure, only need to release its nucleic acid, and then can be detected by nucleic acid detection steps such as NT-PCR and probe testing of nucleic acids If the virus sample has characteristic nucleic acid, it can be judged whether the virus test of the sampling object is positive or infected. Biological virus is a kind of microorganism with simple structure composed of nucleic acid DNA or RNA plus protein. If the sample contains virus characteristic nucleic acid, it can be judged that the sample is infected by the virus.   Desheng Physical and Chemical Performance Testing Laboratory   Since the virus is inactivated without culturing the virus, the first thing that is needed is to cleave and inactivate the virus and destroy its membrane protein to release nucleic acid. Usually, the prepared virus transport media is added with a cracking salt. The cracking salts used by different companies may be different, including guanidine hydrochloride, guanidine isothiocyanate, etc. The essential role is the same, which is to split the viral membrane protein and extract the encapsulated viral nucleic acid. When sampling, the nucleic acid cannot usually be detected immediately. The released nucleic acid will be degraded by contact with RNase in the air. Therefore, an RNase inhibitor needs to be added to the storage solution to inhibit its catalytic RNA degradation. In addition, you need to add Tris buffer and EDTA to maintain the pH of the environment where the nucleic acid is located. Use the characteristics of EDTA to complex metal ions such as calcium, magnesium, iron, etc., to prevent metal ions from activating proteases, reduce the impact on nucleic acid quality, and improve nucleic acid. Stability, extend the storage time.   Use deionized water when configuring the virus transport media, or use ultrapure water for special test requirements. The configuration is similar to our usual configuration of biological buffer, but the temperature requirements are stricter, and the temperature must be kept low during storage and transportation. The virus transport media involves virus sampling and nucleic acid detection, and it must be treated with rigour. After configuration, it needs to be tested for virus inactivation and positive sample detection rate.   The preparation of the virus transport media seems simple, but it is not sloppy for the actual production. It must ensure that the virus is inactivated and loses its infectivity, and it must inhibit the degradation of nucleic acid by RNase. Any failure to do a good job will affect the final detection. Desheng Technology organized scientific researchers to consult materials, consult experts, repeat experiments, and verify by multiple parties, and finally successfully developed a virus transport meida for the new coronavirus.

HEPES塩基配列の分解性を利用しています.HEPES特定のpH値で過剰な金属に,そしてHEPES-NaOH還元方法を使用して,金ナノ粒子を準備します.この方法は操作が簡単で,反応が速く,制御が簡単で,副産物が少なく,環境に優しい.   金のナノ粒子の製造   1濃度0.05〜10 mmol/Lの塩素酸溶液を準備する.   2準備するHEPES5〜50 mmol/Lの濃度のバッファで,HEPESバッファのPH値をナトリウムヒドロキシードで7.0〜8.0に調整する.   3表面活性剤を加えるHEPESステップ2で 1-2 mmol/L の濃度の表面活性剤溶液を調製するために調製されたバッファ   4ステップ3で準備した表面活性剤溶液を反応タンクに挿入します.ステップ1で準備した塩素酸溶液は,塩素酸溶液と表面活性剤溶液のモラー比率1に従って反応タンクにゆっくりと加えます.1 - 110反応は5〜30分間続き,ナノゴールドコロイドを含む混合物を得ます.   5ステップ4で準備された混合物は乾燥し,ナノゴールド粒子を得るように浄化されます.   取っているHEPES構成方法は次のとおりです. まず,2.38 kgのHEPES溶液のpH値はpHメーターを使って約5.4で,その後0.1mol/L のナトリウムヒドロキシード溶液をゆっくりと加え,pHが7に調整されるまで継続的に混ぜます.4最後に,離子化水を200Lに追加します.   準備するHEPES   メソッド1   溶媒として1,2-ディクロロエタン,ヒドロキシエチルピペラジン (5.00g,0.02mol),カリウム炭酸塩Kを使用する2CO3(6.00g,0.04mol),50mLの1,2-ディクロロエタンを,機械的な混ぜ合わせと温度計を備えた100mLの3ポートボトルに加えました.熱点 85°C)反応を停止し,フィルタリング塩をエチルアセタート (EA) 200 ml で洗い,フィルタを乾燥させ,2.6 g のHEPES固体を得ました.   2 方法   11.0g (84.5mmol) 無水ナトリウム硫酸 27.0mL ((343.6mmol) ディクロロエタン 120mL 水 110mL エタノール50mgの銅粉末が3つのボトルに加えられ,磁気調動器と反流凝縮管が次々に使用されました.油浴は熱され,反流まで熱された.22時間反流後,反応液は,すべての白い固体が沉着するまで水を取り除くために低圧で蒸発された.この固体は主に製品で構成されています反応していない原材料と塩分が生成されました. 固体と500mlのエタノールを1Lのコックに入れて,40分間反流のために加熱しました. 熱いままに排水しフィルタを濾し,フィルタを冷却させました.0°Cで一晩放置する排水フィルタリングと真空乾燥により,11.40gと81.0%の収穫率でフラーク結晶化が結果となった.   ナトリウムクロロエチル硫酸塩 (15.10 g,0.08 mol),ヒドロキシエチルピペラジン (9.88 g,0.075 mol),60 mlの水と油浴を磁気混ぜた4つのボトルに追加した.逆流凝縮管と温度計で105°Cで反応を混ぜる反応が進行するにつれて,反応溶液のpHは低下し,pHを約9で制御するために,水溶液5mol/LNaOHを滴滴に追加しました.合計15mlが加えられ,反応は5時間続いた..   反応の終わりに,反応溶液を水で500mLに稀釋し,上部イオン交換樹脂柱 (約500g) を淡化して浄化した.コラムにすべての反応溶液をロードした後排水液のpHが6になるまで蒸留水で洗い,その後1mol/Lのアンモニア水で洗い,製品点 (pH約5-9) を含む排水液をTLC検出のために収集した.ローータリー蒸発は150mlに集中した活性炭の脱色を加え,油浴は110°Cで,加熱し,0.5h間混ぜ,フィルタリング,フィルタレートの回転乾燥,エタノールの50mLを加え,熱リフルクス 0.5h間,熱フィルタリング,乾燥した後に得られる白い固体は8.63Gでした. フィルタートは氷河酸乙酸を滴り,pHを5に調整し,夜間0°Cで冷却し,フィルタリングしました. 乾燥した後に得られる固体は2.80Gでした.合計11件.43gのHEPES固体が64.5%の出力で得られた.   10mmol/L の調製方法HEPESバッファーは以下のとおりです. 2.383gのHEPESを正確に重量化し,1Lに恒定体量の新鮮な蒸発水を3回加え,フィルタリングで不妊化し,サブパッケージング後に4°Cで保管します.細胞培養基に添加されたときにバッファとして使用する場合培養基を光から遠ざけることが推奨されます.   Hubei New Deshengは14年の研究開発と生産経験を持つ生化学原材料の製造業者です.,化学発光反応剤,血液採取用添加物,染色体基質,酵素製剤,抗原抗体など

With the development of the times, people pay more attention to the quality of life, home is a warm and comfortable place for everyone to enjoy, but many decoration companies in order to save costs in the choice of paint is not satisfactory.Therefore, in response to increasingly stringent environmental regulations, water-dispersible polyisocyanates have shown importance in various applications in recent years.   At present, water-dispersible polyisocyanates in most applications are non-ionic hydrophilic modification by polyethers.Although this hydrophilic modified polyisocyanate has gained wide market recognition in most applications, it also has certain drawbacks, such as its high viscosity, which requires a considerable shear force to be applied in the construction process to uniformly introduce it into the aqueous medium.In order to avoid these shortcomings, this also gives CAPS an excellent opportunity.Let it find its location in new coatings.   CAPS in Packaging   Ion-modified groups include carboxyl group, sulfuric acid group, hydroxyl group, hydroxy sulfonic acid, etc., but there are still some defects, such as carboxyl-modified polymers are prone to gelation, hydroxy sulfonic acid-modified products are obviously yellow in color, etc.Later, Bayer reported 3-(cyclohexylamino)-propanesulfonic acid (CAPS)-modified polyisocyanate in its patent CN1429240A. The study found that CAPS-modified polyisocyanate could be finely dispersed in water and the product was stable in storage.CAPS-modified polyisocyanate exhibits certain advantages over other ionic or non-ionic modified products.   1. 3-(cyclohexylamino)-1-propane sulfonic acid (CAPS) reacts with aliphatic polyisocyanates (the former is a zwitterionic aminosulfonate) under mild conditions and in the presence of tertiary amine neutralizers, and the resulting urea sulfonate derivatives are excellent emulsifiers.Regardless of salt forming groups, CAPS-modified polyisocyanates have good storage stability and are not turbid.   Even if they contain fewer sulfonate groups, they can get well dispersed emulsions in water.A series of ionized modified polyisocyanates can be obtained for use in various environmentally friendly high quality waterborne two-component polyurethane coatings.These coatings are comparable to general solvent-based coatings in terms of dry, curing and chemical resistance.The new regulations require further reduction of VOC (volatile organic compounds), and the use of these crosslinkers will definitely increase in the future. Compared with solvent-based coatings, they will not lead to a decrease in paint film quality.   2. Closed water-dispersible polyisocyanate curing agent: Closed water-dispersible polyisocyanate curing agent is a kind of closed polyisocyanate curing agent that is hydrophilically modified so that such products can be dispersed in aqueous resin system.Under the condition of high temperature baking, the blocking agent will be unblocked from the system, releasing isocyanate groups, which react with hydroxyl groups.   3. Closed water dispersible polyisocyanate curing agent is mainly used as crosslinking agent in high temperature baking system.At present, the main use is in the Mid-coating of automobile original paint, in addition, there are also some applications in water-based industrial paint.Closed water dispersible polyisocyanate curing agent can also be used with melamine curing agent to reduce costs,and closed water dispersible polyisocyanate curing agent to improve performance.   CAPS is used as a biological buffer in addition to new materials and coatings, in biochemical diagnostic kits, DNA/RNA extraction kits and PCR diagnostic kits, and in buffer solutions for enzymatic chemistry and HPLC separation of alkaline drugs.  

Trimethylolaminomethane, CAS77-86-1, commonly known as Tris, also known as tromethamine, is a very commonly used reagent, which is widely used in industrial synthesis, biochemical testing, and biopharmaceutical fields; The virus transport media sample tube belongs to an important raw material of its configuration solution.   Trismethylaminomethane Tris molecular structure contains one amino group and three alcoholic hydroxyl groups. The amino group and three methylol groups form a methane-like tetrahedral structure around the central carbon atom. Due to the presence of amino groups, Tris is weakly basic in aqueous solution. The amino group can be used as a coordination group to form Tris salts with many acids. Commonly, Tris-HCl, TEA, TEB, Tris glycine, and Tris phosphoric acid are also used. The raw materials used in the virus transport media are Tris and EDTA. The actual TE buffer is Tris plus EDTA.   Tris powder in drum   Adding Tris to the virus transport meida can first maintain the pH value of the sampled sample and act as a biological buffer. The virus and its nucleic acid are relatively sensitive to the environmental pH. The nucleic acid (DNA, RNA) is easily hydrolyzed in acidic solution. It is more stable in neutral or weak alkaline solution. The Tris hydroxymethylaminomethane, PH buffer range: 7.0-9.0, can maintain the stability of the nucleic acid released after the sample to be cleaved to avoid degradation of the nucleic acid, increase the concentration and purity of the nucleic acid, and help to improve the quality of the nucleic acid To ensure the accuracy of subsequent nucleic acid detection and analysis operations.   Tris buffer is a weak alkaline solution. In such a solution, DNA will be deprotonated to improve its solubility. Therefore, Tris buffer is generally used in the dissolution of nucleic acids and nucleic acid extraction. It should be noted that because it is alkaline when disposing the solution, it will absorb carbon dioxide gas that can generate carbon dioxide in part of the air, so the cap of the bottle containing the Tris solution needs to be tightly capped. In addition, the Tris solution is sensitive to temperature. For every increase of 1 degree, the pH value drops by 0.03, and it needs to be maintained at room temperature during configuration.   Tris buffer is more and more widely used, and even has a tendency to exceed phosphate buffer. Because it does not react with calcium, magnesium and heavy metal ions, its performance is superior to phosphate buffer in many aspects. Desheng's products related to Tris include Tris reagent, Tris hydrochloric acid, TEA/TEB electrophoresis electrophoresis gel, etc., which are superior in quality and low in price.  

伝染病の影響が原因で、新しいcoronavirusのトピックは私達の毎日のトピックになりました。最近、ウーハンは国民の核酸テストを実行しました。核酸の検出に関しては、私達はDeshengによって開発され、作り出されたウイルスの輸送媒体述べています。それはどんな役割を核酸の検出で担いますか。               現在、2つのタイプのウイルスがあります 輸送媒体:不活性にされ、活動化させる タイプ。               ウイルスの見本抽出の綿棒はウイルスの病気の急速な検出に使用することができるPCRと結合されるウイルスの見本抽出の共通方法です。但し、あらゆるサンプル コレクションの場所がPCRの検出を遂行できません従って集められたウイルスの綿棒のサンプル、従って綿棒を運ぶことは必要です ウイルスの輸送媒体 生じました。               Desheng』s ウイルスの輸送媒体             異なった検出の為に、別 ウイルスの輸送媒体使用されるsの必要性。現在、広く利用された2 輸送媒体 自身の特徴を持って下さい。検出の異なった条件およびウイルスの検出の異なった実験室の状態を満たすためには、別を見本抽出することは必要です 輸送媒体。               Virusの輸送媒体 （不活性にされたタイプ）サンプルにinfectivityを失わせるlysateの使用によって呼吸の病原体をすぐに不活性にし、維持するのに使用することができます。不活性にされたサンプルはいろいろなウイルスDNA/RNAの抽出のキット、M3と一致させることができます2核酸の急速な抽出のための/M96の核酸の抽出器、および急速な検出のための呼吸の病原体PCRの検出のキット。特定性の感受性は影響を受けていません。               Virusの輸送媒体 （活動化させたタイプ) かせの液体の基盤、ゲンタマイシン、菌類の抗生物質、BSA （v）、cryoprotectants、生物的緩衝およびアミノ酸を含んでいます。多数の抗生物質の組合せは反細菌および反菌類の効果をもたらします;BSAは、蛋白質の安定装置のような、分解することを困難にし、ウイルスの完全性を保障するウイルスの蛋白質の貝の保護フィルムを形作ることができます;かせの緩衝によって組み立てられる中立環境はウイルスの生存期間および伝染の安定性を高めるのを助けます。 活動化させる ウイルスの輸送媒体 通常臨床インフルエンザ、鳥インフルエンザのコレクションそして交通機関のために使用されます（のような H7N9）の手-フィート-病気、はしかおよびマイコプラズマ、Ureaplasmaおよびクラミジア言って下さい。               Deshengの技術はR & Dそして生産にの託されました ウイルスの輸送媒体、 c伝染病の再開以来のarbomerそして他のプロダクトは、および進歩の勝利を達成しました。使用の後の顧客の断言は私達へ大きい奨励です!私達はよりよい開発および顧客の信頼のだけ目前の利害のために私達のプロダクトを、ない、よくし続けます。                    

Virus transport media is a kind of liquid to protect the tested substance of virus by immersing the virus sample on the sampling swab in the sampling tube. It is generally divided into two types: one is activated type, which can protect the protein and nucleic acid of virus; the other is inactivated type, which usually contains the lysate of inactivated virus, which can lyse the protein and protect the nucleic acid.   Therefore, the virus transport media added with lysed salt is an inactivated virus transport media. The main purpose of the contained Tris, lysed salt, EDTA, etc. is to lyse the nucleic acid and release the nucleic acid, so as to carry out by subsequent real-time fluorescent RT-PCR Nucleic acid detection, to determine whether the sample contains viral characteristic nucleic acid, that is, whether it is infected with a virus.   Inactivated and activated virus transport media   Nucleic acid is a biological macromolecular compound composed of many nucleotides, including deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). It is one of the most basic substances in life. The virus has a single structure and contains only one kind of nucleic acid and protein, so when the characteristic nucleic acid is detected, the virus is detected. Viruses are parasitic organisms and cannot survive in vitro after sampling. If they cannot be detected in time, they need to be put into the virus transport media. In order to protect the security of the virus detection environment, it is necessary to add a lysed salt to inactivate the virus and release the nucleic acid that can be detected.   Guanidine is a nitrogen-containing organic compound, also known as "iminourea", "imicarbazide", and "carbamidine". Cleavage salts are strong inhibitors of nucleases and facilitate the extraction of complete RNA from RNASE-rich tissues. The lysed salt can not only quickly destroy the cell membrane, but also denature the protein, so that the protein is denatured and precipitated, so that the nucleic acid can get rid of the protein. The inactivated virus transport media containing lysed salt can fully and effectively lyse the cell, so that the nucleic acid in the cell can be fully released, and a higher concentration of nucleic acid is obtained, which is conducive to improving the quality of the nucleic acid and ensuring the accuracy of subsequent operations.   In addition to the inactivated virus transport media, Desheng developed and produced a activated virus transport media, which not only saves the integrity of the virus, but also has a higher detection rate. It can also be used for other research besides nucleic acid detection. The inactivated virus transport media is safer to use, the operating environment requirements are not so strict, and each has its own advantages.

Tris (trihydroxymethylaminomethane) is a weak base with a pKa of 8.1 at room temperature of 25℃ and an effective buffer range of pH 7.0 ~ 9.2.The pH of aqueous solution of Tris alkali is about 10.5. Hydrochloric acid is generally added to adjust the pH value to the desired value, so that the buffer of this pH value can be obtained.Since Tris buffer is a weakly alkaline solution, DNA will be deprotonated in such a solution, thus improving its solubility.If the pH-adjusted acid solution is replaced with acetic acid, a "TAE buffer" (Tris/Acetate/EDTA) is obtained, while a "TBE buffer" (Tris/Borate/EDTA) is obtained by replacing it with boric acid.These two buffers are commonly used in nucleic acid electrophoresis experiments.TAE, TBE, etc. prepared by Tris are the most commonly used reagents for DNA electrophoresis, and TE (pH 8.0) is mainly used to dissolve DNA.(TE is a combination of Tris and EDTA.)1MTris-HCl6.8 and 1.5MTris-HCl8.8 are the most commonly used reagents for SDS-PAGE.   TRIS reagent   Among the conventional operations of genetic engineering, agarose gel electrophoresis is the most widely used.Agarose gel electrophoresis is a conventional method for separating, identifying and purifying DNA fragments.It usually uses a horizontal electrophoresis device to electrophoresis under an electric field with constant intensity and direction.DNA molecules are negatively charged in gel buffers (generally alkaline) and migrate from negative to positive electrodes in an electric field.The rate of DNA molecule migration is dependent on the size and conformation of the molecule.The influence of electric field strength and direction, base composition, temperature and embedded dyes, etc.     Operation process: ① TE buffer: (10 mmol/L Tris-HCl, 1 mmol/L EDTA) Electrophoresis buffer (50XTAE)   ② Electrophoresis buffer (50XTAE): Tris 242g, glacial acetic acid 57.1ml, EDTA (0.5mol/L pH 8.0) 100ml Dilute 50 times with distilled water when used.   ③ Sample buffer (6X): 0.25% bromophenol blue, 0.25% xylene blue, 40% (W/V) sucrose   ④ STET buffer (pH 8.0) (8% sucrose, 0.5% Triton, 50 mmol/L EDTA, 10 mmol/L Tris) 1) Measure 100 ml of TAE electrophoresis solution, add 0.7 g of agarose, mix well, place in microwave oven, heat for 3 minutes, fully dissolve agarose. 2) Close the clean and dry electrophoretic plate with sterilized rubber and seal the edge with a small amount of agarose solution.Place the comb and adjust the distance between the bottom edge of the comb and the electrophoresis plate, generally 1-2 mm is appropriate. 3) When the dissolved agarose solution is cooled to about 50C, add 5 M L of ethidium bromide, and the final concentration of ethidium bromide is 1.0 m g/m L. After mixing, pour the agarose solution into the electrophoresis plate and keep it still without moving. 4) After the gel has completely solidified (30-45 minutes at room temperature), gently remove the comb, remove the tape, and place the gel in the electrophoresis pad. 5) In the electrophoresis process, electrophoresis solution (1'TAE) was added to cover the agarose gel surface with about 1-2 mm electrophoresis solution.

3-(cyclohexylamine)-1-propanesulfonic acid, also called CAPS, is a kind of biological buffer, which is commonly used in biochemical diagnostic kit, DNA/RNA extraction kit and PCR diagnostic kit. The buffer used for enzyme chemistry and HPLC separation of basic drugs is relatively stable, with pKa value of 10.4 and pH buffer range of 9.7-11.1 at 25℃. It is widely used in biochemical analysis and in vitro diagnosis.   CAPS in carton   In addition to the biochemical industry, CAPS is widely used in the following fields:   1. CAPS is widely used as biological buffer: in biochemical diagnostic kit, DNA/RNA extraction kit and PCR diagnostic kit; in enzyme chemistry and HPLC buffer for separation of basic drugs. When caps is used as biological buffer, it needs better purity. Generally, it needs analytical purity. When it is used in industry, the purity requirements are relatively low. However, different treatments should be made for different applications.   2. In industry, CAPS is used for new coatings and materials. CAPS is also the raw material for manufacturing welding materials, air conditioning equipment and lithium metal.   3. It is used as analytical reagent, heat exchange carrier and pharmaceutical industry. It is used for air conditioning, fireworks, dry batteries and lithium metal, as flux and desiccant.   4. For coating industry, it is used as curing agent of water-based isocyanate. The water-based isocyanate curing agent can coexist with resins containing active groups (hydroxyl, carboxyl, amino, epoxy, etc.) for a long time at room temperature.   CAPS has such a wide range of uses and many manufacturers. When selecting manufacturers, attention should be paid to identify qualified manufacturers and avoid intermediaries to make profits from them. Hubei New Desheng Materials Technology Co., Ltd. is located in Guanggu United Science and Technology City C8-2, Gedian Development Zone, Ezhou City, Hubei Province. It has 14 years of research and development and production experience in the field of biochemistry and specializes in the production of biological buffers.The enterprise is ISO 9001 quality management system certification approved and has a good reputation in the industry. It is a trusted manufacturer of biochemical raw materials. It is absolutely wise for you to choose Desheng.

ポリウレタンコーティングは,1960年代から普及し始めたコーティング技術の一種である.環境保護法や規制の要求がますます厳しくなるため,環境に優しい水分散型ポリアイソシアナートは,近年,様々な応用分野において重要であることが示されています.ポリエーテル改変型ポリアイソシアナートは広く使用されていますが,それらはすべて一つの基本的な欠点を持っています.特に,水中での二成分ポリウレタンコーティングの交差結合剤として使用する場合十分な分散を保証するために,より高いポリエーテル含有量が必要であり,これは長い乾燥時間と長続きする水利性をもたらします. これは 欠陥が解決されました.キャップス(3- ((サイクロヘキシラミン) - 1-プロパネスルフォン酸) 改変されたポリアイソシアナート.           C についてAPS       C についてAPS (アンフォテリック硫酸塩酸) は,軽度の条件下および三次アミンの中和剤の存在下でアリファティックポリアイソシアナートと反応する.硫酸ウレア誘導体は優れた乳化剤である.塩を形成するグループの影響を考慮せずに,キャップス改変されたポリイソシアナートは保存安定性が非常に良く,最終製品は曇りません. 硫酸塩群が少ない場合でも,溶液は水中でもあり 溶液は散布状態が良好ですこのようにして,様々な環境に優しい高品質の二成分ポリウレタンコーティングに使用できる一連のイオン改変ポリイソシアナートが得られます.   これらのコーティングは,乾燥性,固化性,化学抵抗性において,一般的溶剤ベースのコーティングに完全に優れています.装飾材料のVOC (揮発性有機化合物) の含有量はさらに減少溶剤ベースのコーティングと比較して,フィルム品質の劣化につながらない.C についてAPS 改変されたポリイソシアナートは,自動車原料塗料,修理塗料,プラスチック塗料,木料塗料,布皮塗料,印刷インク産業などに広く使用されており,優れた性能があります.市場見通しは明らかだ.           準備するキャップス改変されたポリイソシアナート       ポリアイソシアネート950gを50gで混ぜたキャップス, 29g のダイメチルサイクロヘキシラミンと 257g の 1-メトキシプロピル-2-イルアセタートを 80 °C で 5 時間乾燥した窒素下で,ポリアイソシアナートはヘキサメチレンダイアイソシアナート (HDI) に基づき,アイソシアナートグループを含んでいる平均的なNCO機能は3である.5モノメア HDI 含有量は 0.1% 粘度 3000mpas です.室温まで冷却した後,無色で透明なポリアイソシアナート溶液を得ることができます.           C についてAPS生物学的バッファとして使用されているため, 化学薬品の製造は,生物化学診断キットに広く使われていますDNA/RNA抽出キットとPCR診断キット企業や個人から,さらに協議を要請することを歓迎します..      

紹介   キャップス, 3- ((サイクロヘキシラミン) --1-プロパネスルフォン酸,有機化学物質,白色結晶粉末,CAS1135-40-6は,生化学診断キットに一般的に使用される生物学的バッファーです.DNA/RNA抽出キットとPCR診断キット酵素化学および基本薬のHPLC分離のためのバッファは,タンパク質配列の膜転送プロセスで広く使用されています.キャップスバッファーはキャップスフィブロネクチンの浄化のために pH を 11.0 に調整します.   CAPS バッファ   主要な活動ポイント   1SDS-PAGE電泳:従来の条件に従って (CAPSシステム: > = 20kD タンパク質; Tris Tricine システム:低分子量タンパク質,高分子量タンパク質); 2メタノール濃度:CAPS電気印刷バッファにおけるメタノール濃度の範囲は0~20%である (メタノール濃度は高く,低分子量タンパク質転送に使用される.メタノール濃度が低く,またはメタノールを含まない場合高分子量タンパク質の転送に使用される) 3.PVDF膜処理:PVDF膜を取り出し,メタノールに数秒浸し,その後CAPS電球バッファに入れる. (注:PVDF膜が操作後に乾燥するのを防ぎなければならない.膜が乾いた場合は,この手順を繰り返します. 4ゲル処理:ゲルゲルを取り出して,CAPSバッファに5〜10分浸す. (注:いくつかの強い基礎タンパク質PI > 9.0を転送する場合,このステップを省略することができます.) 5フィルター紙とスポンジを電波ブッファーに浸し,その後スポンジ,フィルター紙,PVDFフィルム,ジェル,フィルター紙とスポンジローティングスロットに入れます 6移動条件: 常圧50V (100-170MA) で室温で0.5-2hで移動します. (注:ゲルとPVDFフィルムの間に泡を排水します.70 KD 以上のタンパク質は,より長い時間に渡って移植されるべきです); 7PVDF膜染料の予備処理:PVDF膜を取り出して,デイオニ化水で洗浄し,メタノールに数秒浸し,その後染料を塗る. 8膜染め:Coomassie ブリアントブルー染め (0. 1% Coomassie ブリアントブルー R-250 を 40% メタノール/1% 乙酸に溶ける) 30〜50 秒間 (最大 1 分)50% メタノールで色を消す (色を消す溶液を頻繁に交換する)完全に離子化水で洗い,その後乾燥   C についてAPS バッファーの準備   1. 10×CAPS(100mmol/L) バッファ溶液は,以下のように調製します:CAPS, 22.13g; 離子化水を900mlに追加し,2mol/L NaOH (約20ml) でpH値を11.0に調整し,1Lに稀释し,4°Cで保存します. 2.CAPS電印バッファ (1×CAPSが10%メタノールを含んでいる) は,次の方法により調製される: 10 × CAPSの200ml; メタノールの200ml; 離子化水の1600ml.   その他の用途   キャップスまた,溶接材料,エアコン設備,製造用原材料の製造に使用される.また,火花火薬の製造に使用される.分析試料として使用される.熱交換器製薬業界でも使用され,空調,火器,乾電池に使用され,流体および乾燥剤としても使用されます.塗料産業における水性アイソシアナートの固化剤に使用されるデシェン社は,CAPS,Tris,Bicine,MOPSなど,高純度 ≥99%,安定したプロセスを含む,様々な生物学的バッファの研究開発と生産に特化しています.企業や個人に対し,さらなる協議を歓迎します..