Luminol 521-31-3: a luminescent tool in protein imprinting

The Origin and Wide Application of Luminol

Luminol is one of the earliest chemiluminescent reagents put into use. Initially, it was mainly used in the field of criminal investigation to detect trace amounts of blood stains, and emerged with high sensitivity. With the continuous deepening of research, its application scope has gradually expanded, and it has now been widely involved in various biochemical detection experiments, among which its application in protein blotting experiments is particularly prominent.

Luminol's luminescent properties

The chemiluminescence reaction of luminol has significant advantages. Its sensitivity is extremely high, even trace amounts of substances can trigger reactions, providing reliable assurance for detection. The experimental operation is simple and easy to implement, and the process is easy to automate, greatly improving the efficiency of the experiment. Moreover, the luminescence of luminol is visible to the naked eye without the need for additional light sources, effectively avoiding interference from background light and making experimental results clearer and more accurate. Luminol reagent can be mixed with hydrogen peroxide solution to produce ECL luminescent solution, which can emit light under the catalysis of peroxidase HRP or POD. It is worth mentioning that the luminescence process of luminol does not consume HRP. As long as HRP does not decompose, ECL luminescent solution can be repeatedly added to make it glow.

Luminol powder

The principle of protein imprinting

Protein imprinting has similarities in principle with DNA imprinting and RNA imprinting, except that the electrophoretic material is replaced from nucleic acid to protein. This technology first carries out electrophoretic separation of total proteins in cells or tissues, and then transfers the separated proteins from the electrophoretic gel to solid phase supports, commonly used solid phase supports are NC membrane or PVDF membrane. Next, specific antibodies are used to detect specific antigens, thereby achieving protein detection. This technology has been widely applied in various fields such as gene expression research at the protein level, antibody activity detection, and early disease diagnosis.

The specific application of luminol in protein blotting

Luminol plays a crucial role in Western blot experiments. The detected or imprinted protein can be labeled with peroxidase, and after adding luminol luminescent reagent, the target protein will emit light, making it convenient for observation and detection. The specific process is as follows: proteins, protein fragments, and peptides are separated by PAGE and transferred to carriers such as nitrocellulose films. Solid phase carriers adsorb proteins through non covalent bonds while ensuring their activity. Proteins bind to antibodies as antigens, react with HRP enzyme labeled secondary antibodies, and finally detect the protein components of specific target gene expression through luminol luminescence. The advantages of luminescent labeling with luminol

In the Western blot detection method, in addition to luminescent labeling with luminol, there are also enzyme labeled substrate colorimetric and isotope labeled autoradiography methods. However, luminol luminescent markers have unique advantages. It has higher sensitivity and can detect trace amounts of proteins, providing more accurate results for experiments. Moreover, the luminescent label of luminol does not contain radioactive substances, making it safer to use and reducing potential hazards to laboratory personnel and the environment. Desheng Company, which has been deeply involved in the field of chemiluminescence reagents for many years, has the ability to produce various luminescent reagents such as luminol, providing strong support for related experiments.

Product packaging

Hubei Xindesheng Material Technology Co., Ltd. specializes in the production of chemiluminescence reagents such as luminol. With advanced production equipment and rich production experience, our professional technical team ensures product quality and can provide good after-sales service and customized services. If you have any related procurement needs in the near future, please click on the official website for more details or contact me directly!