Acridine ester: a high-quality chemiluminescence reagent in the field of in vitro diagnostics

In the continuous development of in vitro diagnostic technology, chemiluminescence immunoassay (CLIA) technology occupies an important position due to its excellent performance, and acridine ester, as the core luminescent reagent of this technology, has shown significant application value.

1, The basic characteristics and development of acridine esters

The molecular structure of acridine ester is based on acridine, and its luminescent material is similar to early glargine. In the practical application of in vitro diagnostics, acridine esters are not a single type, but continuously innovate and develop on the basis of traditional acridine ester AE-NHS. At present, nearly ten types have been derived, of which at least six or more have been successfully applied to chemiluminescence assay kits. These acridine ester luminescent reagents mainly cover two types: acridine ester and acridine sulfonamide, and there are also other new types of acridine esters under continuous research and development. With the advancement of research and development, the overall performance of acridine esters is continuously optimized, which can better meet the needs of in vitro diagnostics. Moreover, the luminescence principles of various acridine esters are basically similar, providing a stable technical basis for their wide application in reagent kits.

Acridine ester powder

2, Significant advantages of acridine ester chemiluminescence assay kit

Chemiluminescent labeled immunoassay (CLIA) uses chemiluminescent agents to directly label antigens, haptens, or antibodies. Acridine substituents are commonly used as labeled chemiluminescent reagents, and their performance varies depending on the substituent. When acridine ester is activated by luminescent reagents (NaOH, H ₂ O ₂), it produces strong direct luminescence, and this luminescence process can be completed within one second, belonging to rapid scintillation luminescence.

The advantages of this type of reagent kit are very prominent. Its chemical reaction process is simple and fast, without the need for additional catalysts, greatly improving detection efficiency. In terms of detection methods, the competitive method is used for small molecule antigens, while the sandwich method is used for large molecule antigens. The detection method has strong specificity. At the same time, there is less non-specific binding and lower background during the detection process of the reagent kit, effectively reducing the influence of interference factors. More importantly, the combination of acridine ester with macromolecules does not result in a decrease in the amount of light produced, which significantly enhances the sensitivity of detection and makes the results more accurate and reliable.

3, The key role of acridine ester labeled complexes

Acridine ester labeled complexes are complexes formed by binding acridine ester to the target substance (such as antibodies, antigens, etc.), also known as acridine labeled complexes. In chemiluminescence immunoassay technology, the quality of acridine labeled complexes plays a decisive role and directly affects the success or failure of the entire analysis technique. Therefore, it is considered a key reagent in this technology. Only by ensuring the good quality of the acridine ester labeled conjugate can the smooth progress of the chemiluminescence immunoassay process be guaranteed, thereby obtaining accurate detection results.

4, Common methods for labeling proteins, antibodies, and antigens with acridine ester

Among the methods for labeling proteins, antibodies, and antigens with acridine ester, the NHS ester (N-hydroxysuccinimide) binding method is widely used. This method allows acridine ester to directly bind to the primary amino group on the protein, and the operation is relatively simple. In addition, the carbodiimide crosslinking method is also one of the commonly used methods, which uses carbodiimide crosslinking agents as bridges to achieve the binding of acridine substituents to the protein to be labeled. These two methods each have their own characteristics, and the appropriate method can be selected according to the actual labeling needs and the characteristics of the target substance, providing technical support for the effective application of acridine esters in in in vitro diagnosis.

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As an advantageous manufacturer of luminescent reagents, Desheng has a history of nearly 20 years since its establishment in 2005, with rich research and development experience. Currently, in addition to the acridine ester series products, the chemical luminescent reagents available for sale include luminol, isoluminol, and luminol monosodium salt. Desheng has professional laboratories and technicians who can provide customized services according to customer requirements. It is your trusted supplier of chemiluminescence reagents. If you have any related procurement needs in the near future, please feel free to contact me!