Analysis of the effect of drying treatment on the anticoagulant effect of EDTA dipotassium 25102-12-9

In the field of clinical testing and blood sample preservation, the anticoagulant effect of dipotassium EDTA in blood collection tubes directly determines the accuracy of test results. As a commonly used anticoagulant, dipotassium EDTA binds with calcium ions in the blood to form a stable chelate, thereby preventing blood clotting. In the production process of blood collection tubes, "spraying EDTA dipotassium solution+drying treatment" is a standardized process. If many factors in this process are not properly controlled, they can directly damage the anticoagulant mechanism of EDTA dipotassium and cause serious interference to subsequent inspection work. Its impact can be analyzed in detail from the following three aspects.

1, Solution evaporation caused by drying: the core cause of anticoagulant concentration imbalance

EDTA dipotassium is often sprayed on the inner wall of blood collection tubes in the form of an aqueous solution. During drying, high temperatures accelerate the evaporation of water, but some EDTA dipotassium molecules will escape with water vapor, especially when the equipment has poor sealing and the airflow is too fast, the evaporation amount is more significant.

EDTA dipotassium powder

The anticoagulant effect is strictly positively correlated with concentration: the concentration decreases due to volatilization, and the ability to bind calcium ions decreases synchronously. If it is below the standard threshold (1.8-2.2mg EDTA dipotassium per milliliter of blood), free calcium ions cannot be completely chelated, which will initiate the coagulation cascade reaction and cause partial coagulation of the blood. This not only increases the blood cell count, but also destroys the plasma components, affecting the accuracy of biochemical indicator detection.

2, Drying temperature out of control: key factor in disrupting the molecular structure of EDTA dipotassium

The stable molecular structure of EDTA dipotassium requires an appropriate temperature. When the drying temperature exceeds 60 ℃, the internal chemical bonds of the molecule break, the spatial structure changes, and the ability to bind calcium ions is irreversibly lost. After cooling, the activity cannot be restored.

In actual production, if the temperature sensor of the equipment malfunctions or the heating elements are unevenly distributed, it will cause local high temperature in the blood collection tube. The affected EDTA dipotassium will form an "ineffective coating" on the tube wall, making it unable to anticoagulate after blood injection and rapidly coagulating in a short period of time. This not only leads to sample scrapping, but may also result in incorrect test results due to failure to detect coagulation in a timely manner, misleading clinical diagnosis.

3, Improper drying time: an important variable causing uneven anticoagulant effects

If the time is too long, it will exacerbate the "migration phenomenon" of EDTA dipotassium, causing molecules to aggregate towards the bottom or edge of the tube, and the content in the middle of the tube wall will sharply decrease. After blood injection, the dense area of anticoagulant can be anticoagulated normally, while the sparse area may coagulate due to insufficient coagulation, forming a "partially coagulated sample". After centrifugation, the separation of serum and blood clots is unclear and the cells rupture, which seriously affects the reliability of the test.

In summary, the temperature, time, and volatilization control of the drying process directly determine the anticoagulant effect of EDTA dipotassium. During production, it is necessary to optimize equipment parameters, strengthen process monitoring, reduce external influences, ensure stable anticoagulant performance of blood collection tubes, and provide accurate blood samples for clinical testing.

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Hubei Xindesheng Material Technology Co., Ltd. has been researching and producing blood collection tube additives such as EDTA dipotassium for nearly 20 years. The performance of various products produced not only meets relevant standards, but also meets the customized needs of customers. If you are interested in our products, please click on the official website to learn more details or contact me!